Descripción
INGREDIENTS | GMS / LITRE | ||
|---|---|---|---|
Casein enzymic | 5.000 | ||
hydrolysate | 2.500 | ||
Yeast extract | 1.000 | ||
Dextrose Agar | 15.000 | ||
Final pH ( at 25ºC) | 7.0+-0 |
GAR PLATE COUNT STANDARD X 100GR
- Plate Count Agar is formulated as described by Buchbinder et al (1) This is recommended by APHA (2-4) and FDA (5).
Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate technique as described below. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile rooms.
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